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For measuring CD38 NAD + ase activity, spinal cord tissue was homogenized in NETN buffer (100 mM NaCl, 1 mM EDTA, 20 mM Tris-HCl, and 0.5% Nonidet P-40) supplemented with 5 mM NaF and protease and phosphatase inhibitors (Tarrago et al., 2018)

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