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On day 7, the cells were stained with anti-GLUT1 (R&D Systems, MAB1418, 1:500) conjugated with Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (1:500, Invitrogen, A32723) and checked the intensity by flow cytometry (LSR Foretessa 5 laser Flow Cytometer, BD, NJ, USA)
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