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Next, we washed sections with PBS and counterstained them with DAPI

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As a selective substrate site-targeting inhibitor of nicotinamide N-methyltransferase (NNMT) — the cytosolic enzyme responsible for the methylation of nicotinamide using S-adenosylmethionine (SAM) as a methyl donor — 5-Amino-1MQ is studied as a tool compound for investigating NNMT enzyme activity, NAD⁺ salvage pathway interactions, and methylation pathway dynamics in controlled in-vitro experimental systems