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Compared to healthy young adults, who have a high proportion of Firmicutes , older adults demonstrate a decrease in Bifidobacteria and an increase in Bacteroidetes , as well as a decline in gut microbial diversity, with a reduction in the relative abundance of core species and an augment in the colonization of opportunistic species ( Betaproteobacteria ) ( Bacteroidetes and Firmicutes are the most common bacteria found in the GM of older individuals (Wu et al., 2021)

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Lastly, the widely used serum cysteine assay method we chose to analyze total serum cysteine using a fluorometric assay kit may not have been sensitive to subtle cysteine changes in the blood after increased milk intake