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Incorporating sex as a biological variable in future studies will therefore be essential to determine whether males and females may benefit differently from antioxidant-based treatments and to address the current imbalance in ASD research

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In vitro pNPG processing assay The standard substrate p -nitrophenyl-β-D-glucuronide ( p NPG) was purchased as a solid (Sigma Aldrich) and resuspended in water to a concentration of 100 mM

This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH