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Then, the SGZ sections were selected and incubated in X-Gal solution containing 40 mM citrate buffer (pH=6) (C7129, Sigma-Aldrich), 5 mM K3Fe (CN)6 (P8131, Sigma-Aldrich), 5 mM K4Fe (CN)6 (P9387, Sigma-Aldrich), 2 mM MgCl 2 (208337, Sigma-Aldrich), 150 mM NaCl (31434, Sigma-Aldrich) and 1 mg ml −1 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) (10703729001, Sigma-Aldrich) in PBS (pH 6)] at 37 °C in the dark for 48 h