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The gut microbiota mediates the anti-seizure effects of the ketogenic diet - PMC

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Immunoblotting Total protein from hMEC was extracted in lysis buffer containing Tris (50 mmol/L, pH 8.0), NaCl (150 mmol/L), Triton X-100 (1%), SDS (0.1%), supplemented with phenylmethylsulfonyl fluoride (PMSF