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After digesting the myocardial tissues with trypsin (Thermo Fisher Scientific, 25300-062) and collagenase type 2 (Worthington Biochemical Corporation, LS004176), the cells were suspended in DMEM (Sigma-Aldrich, D5796) containing 10% FBS (HyClone Laboratories, SH30910.03) and 1% penicillin/streptomycin (P/S
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along with it, they identified four QTLs associated with Met content which together explained 57.3% variation among genotypes
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