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lysis buffer 5 mM imodazole in lysis buffer imidazole 5 mM and 20 mM, in lysis buffer Bound mt-hNNMT was eluted from the column with lysis buffer and 150 mM imidazole, 200 mM salt, and 5% glycerol in 1 ml aliquots

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Freezing is only appropriate for unreconstituted lyophilized powder