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ROS levels increase either by up-regulation of prooxidant enzyme activity, (de−) regulation of electron flow or down-regulation of the antioxidant system
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ERR target-gene expression (for example, Ppargc1a, Tfam, OXPHOS subunits), oxygen-consumption rate in cells, and, in vivo, indirect calorimetry parameters (energy expenditure, RER), plus mitochondrial DNA and cytochrome-c content in muscle
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