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It has been shown in the literature (45, 46) that nuclear factor erythroid 2-related factor 2/Kelch-like ECH-associated protein 1 (Nrf2-keap1) is the main mechanism of resistance to oxidative stress, and demethylation of keap1 promoter activates the expression of Keap1 protein, which promotes the degradation of Nrf2 proteasome and attenuates Nrf2 activity, leading to the reduction of resistance to oxidative stress and ultimately causing the cataract occurrence
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In a real situation, coffee enema users might perform this procedure using different types of coffee with wide variation in coffee concentrations/volumes as well as retaining durations
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