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Following incubation, media of treated cells were analyzed for cytokine levels using a Human Grp I Cytokine 7-Plex Panel kit (Bio-Rad) specific for interleukin (IL)-1 β , IL-6, IL-8, IL-10, IL-15, TNF- α , and eotaxin, using a Bio-Plex 200 System (Bio-Rad) with Bio-Plex Manager software in accordance with the manufacturer's instructions

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Watch the video Administering a Subcutaneous Injection developed by Renée Anderson and Wendy McKenzie (2018) of TRU School of Nursing