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By directly comparing the cellular uptake of anti-FITC-594 mediated by 50 nM Ab-FA and 200 nM FA-FITC, a significantly greater amount of internalized anti-FITC-594 was observed in the cells treated with Ab-FA than FA-FITC, suggesting that antibody-based degrader Ab-FA has higher uptake efficiency over FA-FITC (Fig

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The transferred PVDF membrane was then closed in 5% bismuth sulfite agar (BSA) for 1 h and incubated overnight at 4 °C with the corresponding primary antibodies, including RNF128 (abcam, ab137088 and ab72533, 1:1000, CA, USA), Beclin1 (CST, #3495, 1:1000), ATG5 (CST, #12994, 1:1000), LC3B (CST, #83506, 1:1000), p62 (CST, #5114, 1:1000), SLC7A11 (Proteintech, 26864-1-AP, 1:1000, MA, USA), GPX4 (Proteintech, 67763-1-Ig, 1:1000), Flag (Proteintech, 66008-4- Ig, 1:1000), Myc (Proteintech, 16286-1-AP, 1:1000), HA (Proteintech, 81290-1-RR, 1:1000), β-actin (Proteintech, 51067-2-AP, 1:5000)