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65 ) (Addgene, 52961) for CRISPR–Cas9 KO or into pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 66 (Addgene, 71236) for CRISPRi knockdown, as previously described 41
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At these sites, laminin-211 secreted from astrocytes binds to dystroglycan, a non-integrin receptor encoded by DAG1 on the surface of DTCs, promoting DTCs quiescence
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