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146 developed rapamycin-loaded poly (lactic-co-glycolic acid) microparticles (RMPs) for treating mice with articular OA and found that RMPs effectively induced autophagy, as evidenced by increased levels of LC3B and reduced the expression of the senescence marker p16INK4a

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Total RNA was extracted from AT2 cells according to the instructions of the Total RNA Extraction Kit (Beijing Solarbio Science & Technology Co., Ltd.)