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Flies were infiltrated with 25% Durcupan epoxy resin to 75% dry acetone for 4 h, 50% Durcupan epoxy resin to 50% dry acetone overnight, 75% Durcupan epoxy resin to 25% dry acetone for 8 h, 90% Durcupan epoxy resin to 10% dry acetone overnight and Durcupan ACM (four times for 8–12 h each) and then embedded in a vacuum oven at 60 °C for 1 week