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Collagenase digestion was used to isolate satellite cells (0.5% collagenase B, 1.2 U/ml Dispase II (Roche) in Ham’s F-10 media (Thermo Scientific)), and enrichment in non-collagen coated flasks before initiation of culture and between passages used to reduce fibroblast content

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Based on published evidence, semaglutide is the far more effective choice for fat loss, and AOD-9604 is now FDA-restricted (Category 2 bulk drug substance) for US compounding