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Cell-bound IFN-γ, CD11b, CD8 and CD4 expression in LLC single tumor cells upon the different treatments was analyzed by flow cytometry using the antibodies: PE/Cy7 anti-mouse IFN-y (Biolegend, 505825), PE-Cy7 Rat Anti-Mouse CD11b (BD Pharmingen, 552850), V500 Rat anti-mouse CD8a, clone 53–6.7 (BD Horizon, 560776), FITC conjugated anti-mouse CD4, clone RM4–5 (Ebioscience, 11-0042-85) according to manufacturer’s instructions
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However, although RAF inhibitors are employed for tumors expressing mutant BRAF, their usefulness is limited by toxicity caused by their paradoxical enhancement of ERK signaling in BRAF wild-type cells (reviewed in [49])
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