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Statistical significance can be achieved with small samples when effect sizes are large, but generalisability remains uncertain

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However, human epidemiological evidence for transgenerational effects remains limited, primarily due to the extended timeframes required for multigenerational studies and the practical challenges of maintaining long-term cohorts across decades ( Low-Dose Chronic Exposure Effects Present Assessment Challenges EEDs frequently exhibit non-monotonic dose-response (NMDR) patterns, where low-dose chronic exposure may produce more pronounced biological effects than acute high-dose exposure